Bernard C. Silvala
University of Tabuk, KSA
Title: Panton – Valentine Leukocidin producing Methicillin Resistant Staphylococcus aureus from three health care centers in Tabuk City – Saudi Arabia
Biography
Biography: Bernard C. Silvala
Abstract
Panton-valentine leukocidin (PV-luk) toxin is a very potent toxin responsible for severe and fatal form of Staphylococcal infections. This study aimed to determine the frequency of PV-luk producing methicillin resistant S. aureus (MRSA) and compare the conventional against real time PCR for identification of MRSA. A total number of 47 MRSA isolates were enrolled in the study. All are identified and typed using VITEK system. DNA was extracted and subject to conventional and real time PCR (RT-PCR) for the detection of mecA, luk-PV, orfX, mecA/mecC SCC/orfX junction genes. Forty-five isolates (95.7%) confirmed as MRSA and 2 isolated showed a mixed infection of Methicillin sensitive S. aureus (MSSA) and Methicillin resistant coagulase negative Staphylococci (MRCoNS). Fifteen out of forty-seven (31.9%) have Panton – Valentine Leukocidin gene (luk-PV) gene. PV-luk was detected in one third of tested MRSA emerging the possibility to have a potent superbug. In addition to, diagnosis of MRSA is highly accurate when using multiplex real time PCR rather than conventional PCR.