Day 2 :
- Use of Nanoparticles in DDS and Newer Methodologies | Analytical strategies for pharmaceutical products
Location: Orlando, USA
Karyn I Cotta
South University, USA
Shreemati Nathibai Damodar Thackersey Women�s University, India
University of Nimes, France
Time : 10:00-10:20
Miyanou Rosales-Hurtado holds a master’s degree in Molecular and Macromolecular Chemistry at the University of Bordeaux, in Talence, France. In 2016, she had done an internship on G-quadruplexes at European Institute of Chemistry and Biology. She is the second author on the article “Design, Synthesis, and Evaluation of 2,9Bis[(substituted-aminomethyl)phenyl]-1,10phenanthroline: Derivatives as G-Quadruplex Ligands.” She is now a Ph.D. student at the University of Nimes (France) under the supervision of Prof. Patrick Meffre and Dr. Zohra Benfodda. Her doctoral research investigates the design, synthesis, and evaluation of new potential agents to combat antibacterial resistance.
Infections caused by multidrugresistant bacteria represent one of the biggest challenges in the medical field. There is an urgent need to develop new antibacterial targets and antibacterial agents to combat multidrug-resistance bacteria. For several years, the main target has been focused on the peptidoglycan biosynthesis pathway which provides a potential route to design novel antibiotic compounds. In fact, most bacteria require either lysine or its biosynthetic precursor, diaminopimelate acid (DAP), as a component of the peptidoglycan layer of the cell wall. Nevertheless, DAP/lysine are not present in mammals, thus, inhibitors of this pathway could provide potential antibacterial agents displaying low mammalian toxicity. In order to fight antibacterial resistance, our work focused on the design and synthesis of new analogs of diaminopimelic acid (DAP) and lysine.
University of Bonn, Germany
Title: The emergence of colistin-resistant bacteria in wastewater from poultry and pig slaughterhouses and their dissemination into surface water
Time : 10:20-10:40
Mykhailo Savin has studied Food Technology at the University of Bonn and got his M.Sc. degree in 2016. From 2016 on, he has been working as a Ph.D. student at the Institute of Animal Sciences at the University of Bonn. He is involved in the BMBF (German Federal Ministry of Education and Research)financed project “HyReKa” (02WRS1377C), where he investigates the occurrence and dissemination of clinically relevant antibioticresistant pathogens from poultry and pig slaughterhouses via wastewater and sewage water treatment plants into surface waters. Based on these results, recommendations for the prevention of dissemination should be formulated.
Background and Objectives: Although colistin is a last resort antibiotic, it is applied regularly in animal production. Its increased use may have triggered the emergence of colistin-resistant bacteria. Hence, the aim was to investigate their occurrence in wastewater from poultry and pig slaughterhouses and to examine their emergence after the treatment process in the in-house and municipal wastewater treatment plants (WWTPs). Materials and Methods: Wastewater samples were taken during 2017-2018 along the production chains in two German pig slaughterhouses (n=53) and in its municipal WWTPs (n=36) as well as in two poultry slaughterhouses (n=72). Samples were screened for colistin-resistant bacteria using EMB medium supplemented with 3.5µg/ml colistin sulfate. The final identification was done by MALDI-TOF-MS. Antimicrobial susceptibility tests were performed by the broth microdilution method. Colistinresistant isolates were screened for the presence of colistin resistance genes (mcr 1-5) by PCR. Results: Colistin-resistant bacteria were more abundant in wastewater from the poultry slaughterhouses. Overall, 41.7% of its samples (n=30/72), including the outflows of the in-house WWTP, were found positive. The percentage of positive samples from the pig slaughterhouses and its mWWTPs was lower at 18.0% (n=16/89). Out of 46 samples, a total of 106 colistin-resistant strains were obtained. Among these, the majority belonged to E. coli (39.6%), followed by E. cloacae complex (28.3%), K. pneumoniae (27.4%) and R. ornithinolytica (4.7%). The mcr-1 gene was detected in 69.0% of the E. coli strains (n=29/42), in 13.8% of the K. pneumoniae strains (n=4/29) and in 3.3% of the strains from the E. cloacae complex (n=1/30). Conclusions: Colistin-resistant bacteria were detected in wastewater throughout the production chains as well as in the effluents and preflooder of the in-house and municipal WWTPs. This could pose a threat to human health and needs to be further investigated.